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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Superbinder based phosphoproteomic landscape revealed PRKCD_pY313 mediates the activation of Src and p38 MAPK to promote TNBC progression

Fig. 4

The expression level of PRKCD_pY313 in breast cancer tissues and cell lines. (A) The functional regions and tyrosine sites structure of PRKCD. (B) The mRNA level of PRKCD in breast cancer tissues based on TCGA samples. (C) Western blots revealed the protein expression level of PRKCD_pY313 in breast cancer tissues based on CPTAC samples. (D) Breast cancer patients with high PRKCD expression level has poor survival based on TCGA databases. (E) The level of PRKCD_pY313 and related kinases in breast cancer and paired far-cancer normal tissues. (F) The level of PRKCD_pY313 and PRKCD in breast cancer cell lines (BT549, MCF7, T47D, MDA-MB-231, SKBR3) and immortalized breast cells (MCF10A). (G) The relative expression of PRKCD after normalization to the signals of GAPDH in Fig. 4F. (H) The relative expression of PRKCD_pY313 after normalization to the signals of PRKCD in Fig. 4F. (I) The relative expression of the phosphorylation level of PRKCD_pY313 and its associated kinase active site after normalization to the signals of their protein in Fig. 4E. FC, fold change; nf, nonfunctional; TNBC, triple negative breast cancer. Mean ± SD, n = 3 per group, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

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Cell Communication and Signaling

ISSN: 1478-811X