Cell Communication and Signaling

Table 1 Fluorescent conjugated antibodies used in the study

From: Immunophenotype profile by flow cytometry reveals different subtypes of extracellular vesicles in porcine seminal plasma

Feature	EV Protein
Feature	CD9	CD63	CD81	HSP90β	CD44	Albumin
Antibody	CD9 Antibody, anti-human	CD63 Antibody, anti-human	CD81 Antibody, anti-human	HSP90β polyclonal antibody	Rat anti mouse CD44	Anti-swine Albumin
Conjugate	PerCP	FITC	APC	PE	FITC	FITC
Species reactivity	Human	Human	Human	Pig and other	Mouse	Pig
Pig cross-reactivity	No¹	No¹	No¹	Yes	Yes	Yes
Manufacturer	Miltenyi Biotec	Miltenyi Biotec	Miltenyi Biotec	Enzo life Sciences	Bio-Rad	Cedarlane Laboratories
Code	130–118-814	130–123-673	130–119-787	ADI-SPA-844PE	MCA4703F	CLFAG16140

Bio-Rad, Hercules, California, USA; Cedarlane Laboratories, Burlington, Canada; Enzo Life Sciences, Farmingdale, NY, USA; Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany
EV extracellular vesicle, APC allophycocyanin, FITC Fluorescein isothiocyanate, PE Phycoerythrin, PerCP Peridinin Chlorophyll Protein
¹Identity and similarity of amino acid sequence alignments between humans and pigs greater than 86% (see text)

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ISSN: 1478-811X

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