Fig. 4
Box plots showing the percentages of porcine seminal plasma (SP) extracellular vesicles (sEVs) expressing HSP90β (A-B) and CD44 (C-D). Seminal EVs were isolated by SEC in two different size subsets (small [S-sEVs] and large [L-sEVs]) and from four different SP sources: the first 10 mL of the sperm rich ejaculate fraction (SRF-P1), the remaining of the SRF (SRF-P2), the post-SRF (PSRF), and from the entire ejaculate (EE). Data from six replicates. Boxes enclose the 25th and 75th percentiles, whiskers extend to the 5th and 95th percentiles, and the line indicates the median. Different asterisks indicate different statistical values (*: P < 0.05; **: P < 0.01; ***: P < 0.001; ****: P < 0.0001)