Fig. 6

Depletion of Akt3 downregulates E-cadherin and NCAM. A The expression of E-cadherin and NCAM was detected in total cell lysates by Western blotting using specific antibodies and GAPDH or β-actin as loading controls. Representative blots of n ≥ 3 independent experiments are shown. For NCAM expression, MiaPaCa-2 and HEK293 cell lysates served as negative and positive control, respectively. B The content of the mRNA of CDH1 and NCAM was determined by qRT-PCR analysis. Relative expression was assessed in relation to RPLP0 and normalized to the expression in the cell clone transduced with a scrambled shRNA [Scr] set to 1. Each sample was analyzed in duplicate. Mean ± SD (n = 3–6, one sample t-test, *** p ≤ 0.001; ** p ≤ 0.01; * p ≤ 0.05) is shown