Fig. 4

DA could bind to E166 residue of α-enolase. A-C In-gel fluorescence visualization of recombinant human aldolase A, α-enolase or PKM2 after labeling with different concentrations of DA-P. D-F In-gel fluorescence visualization of recombinant human aldolase A, α-enolase and PKM2 labeled by DA-P in the presence or absence of the competitors DA or IAA. G MS/MS spectrum of human α-enolase treated with 100 μM DA for 60 min. The amino acid E highlighted in red represents the glutamic acid bound to DA. H A binding model of DA with human α-enolase predicted by molecular docking. Fluo, fluorescence; CBB, coomassie brilliant blue