Fig. 3

DA suppresses the enzymatic activities of α-enolase and PKM2. A, B Immuno-blots showing the levels of endogenous α-enolase and PKM2 after incubation with indicated concentrations of DA for 24 h. C, D Measurement of the catalytic activities of recombinant α-enolase and PKM2 in the presence of indicated concentrations of DA treatment. E Quantification of relative ATP levels in MN9D cells treated with 0 μM, 200 μM, 400 μM or 800 μM of DA for 24 h. F Measurement of ATP levels in the DA-injected mouse striatum. ATP were measured one day following an intrastriatal injection of DA (0.3 μM). G Flow cytometric analysis of JC-1 aggregates in MN9D cells treated with DA for 24 h. H Representative flow cytometric plots and quantification results of apoptotic cells after 24 h of DA exposure. Error bar, mean ± s.d.;. n.s., not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001