Fig. 7
Phosphorylation of PRDX6 mediates NOX2 activation by regulating its translocation and gaining iPLA2 activity in astrocytes. a Quantification of ROS. b DCFH-DA staninig. c Western blot analysis of membranous PRDX6, NOX2, P22phox, P47phox, Rac1, and total PRDX6 levels. d Immunoprecipitation and immunoblot analyses of the interaction between P22phox, P47phox, Rac1 and P67phox. e PRDX6 peptides. f Immunoprecipitation and immunoblotting analyses of PRDX6 phosphorylation. g Western blot analysis of membranous PRDX6, total PRDX6, and p-PRDX6 levels. Values are mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001