Fig. 5

Inhibition of astrocytic PRDX6-iPLA2 influences microglia/infiltrated macrophages polarization through mediating the generation of ROS. a DCFH-DA staninig. b Quantification of ROS. c Immunocytochemistry of Iba-1 staining for microglia. CD16 and CD206 expression were detected by immunohistochemistry in BV2 cells. Cell nuclei were stained with DAPI (× 400). d Histogram reveals fluorescence intensity of CD16 and CD206. e Quantification of iNOS, IL-1β, TNF-α, Arg-1, IL10 and IL-4 in BV2 cells by ELISA. Values are mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001