Fig. 4From: PRDX6-iPLA2 aggravates neuroinflammation after ischemic stroke via regulating astrocytes-induced M1 microglia Inhibition of astrocytic PRDX6-iPLA2 influences microglia/infiltrated macrophages polarization. a Western blot analysis of PRDX6 in CTX-TNA2 cells or BV2 cells. b iPLA2 activity in CTX-TNA2 cells or BV2 cells. GPx activity in CTX-TNA2 cells or BV2 cells. c Immunocytochemistry of Iba-1 staining for microglia. CD16 and CD206 expression were detected by immunohistochemistry in BV2 cells. Cell nuclei were stained with DAPI (× 400). d Histogram reveals fluorescence intensity of CD16 and CD206. e Quantification of iNOS, IL-1β, TNF-α, Arg-1, IL10 and IL-4 in BV2 cells by ELISA. Values are mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001Back to article page