Fig. 6

PDIA3P1 is a direct transcriptional target of the OCT4. A PDIA3P1 mRNA was measured by qRT‐PCR in cells with transfected OCT4 siRNA (***p < 0.001, student t-test). B PDIA3P1 mRNA was measured by qRT‐PCR in cells with transfected OCT4 Cdna (***p < 0.001, student t-test).C PDIA3P1 mRNA was measured by qRT‐PCR in cells with co-transfected with OCT4 cDNA and WWP2 cDNA (**p < 0.01,***p < 0.001, two-sided Student’s t-test). D, E PDIA3P1 promoter luciferase activities were measured after silencing of OCT4 (D) or overexpressed OCT4 (E) (***p < 0.001, student t-test). F Schematic representation of four different lengths of the PDIA3P1 promoter normal construct and three OCT4 mutant constructs. G The recognition motif of OCT4 obtained from the JASPAR. H, I Cells were transiently cotransfected with OCT4 siRNA (H) or OCT4 cDNA (I), four different lengths of the PDIA3P1 promoter luciferase reporter, and luciferase activity was determined (ns, no significance; **p < 0.01, ***p < 0.001, student t-test). J, K Cells were transiently cotransfected with OCT4 siRNA (J) or OCT4 cDNA (K), three mutant OCT4 binding site constructs of PDIA3P1 promoter luciferase reporter, and luciferase activity was determined (ns, no significance; **p < 0.01, ***p < 0.001, student t-test). L Schematic representation of Cleavage under targets and tagmentation (CUT&Tag). M Eca-109 cells were subjected to CUT&Tag assays by using anti‐OCT4 antibodies or control IgG. (Left panel) Standard PCR products were run and scanned. (Right panel) qRT‐PCR results were quantified and are indicated (***p < 0.001, student t-test). All, the datas represent the mean ± S.D. of triplicates