Fig. 1

PDIA3P1 is highly expressed and required for malignant behaviors in ESCC cells. A PDIA3P1 expression level in esophageal cancer (n = 182) compared with normal tissues (n = 286) in GEPIA database (*p < 0.05, student t-test). B Analysis of PDIA3P1 gene expression in GEO datasets (GSE111011). C qRT-PCR was used to detect the relative expression of PDIA3P1 in 26 paired ESCC tissues and non-tumor specimens (***p < 0.001, paired t-test). D Expression levels of PDIA3P1 were compared between patients in the TNM stage I/II group (n = 15) and the TNM III/IV group (n = 11) (*p < 0.01, student t-test). E PDIA3P1 expression levels were assessed in the T1 group (n = 8), the T2 group (n = 9) and T3 group (n = 9) (*p < 0.05, ***p < 0.001, for difference from the T1 grade by ANOVA with Dunnett's correction for multiple comparisons). F Expression levels of PDIA3P1 in normal cell line HEEC and five ESCC cell lines (KYSE-30, KYSE-150, KYSE-520, TE-1 and Eca-109) were examined using qRT-PCR (**p < 0.01,***p < 0.001, for difference from the HEEC cells by ANOVA with Dunnett's correction for multiple comparisons). G The expression of PDIA3P1 was knocked down using two siRNAs in TE-1 and Eca-109 cells (***p < 0.001, for difference from the transfected with si-NC by ANOVA with Dunnett's correction for multiple comparisons). H Overexpression of PDIA3P1 by transfected expression plasmid in KYSE-30 and KYSE-150 cells (***p < 0.001, student t-test). I CCK-8 assay of the cell proliferation after knocking down PDIA3P1 in ESCC cells (**p < 0.01, ***p < 0.001, for difference from the transfected with si-NC by ANOVA with Dunnett's correction for multiple comparisons). J Colony formation assay after silencing of PDIA3P1 in TE-1 and Eca-109 cells (***p < 0.001, student t-test). K EdU assays were performed to assess the proliferative ability of ESCC cells with PDIA3P1 knockdown (**p < 0.01, ***p < 0.001, student t-test). L The apoptosis analysis of PDIA3P1 knockdown by flow cytometry (***p < 0.001, student t-test). M Transwell assays were conducted to examine the effects of PDIA3P1 knockdown on ESCC cells migration and invasion (**p < 0.01, ***p < 0.001, student t-test). N Western blot shows expression levels of E-Cadherin, N-Cadherin, Vimentin and Snail after transfection with PDIA3P1 siRNA. F-M, these data represent the mean ± S.D. of triplicates