Fig. 5

The specific epigenetic mechanism of low corticosterone (CORT) downregulating insulin-like growth factor I (IGF1) expression in vivo and in vitro. A The histone 3 lysine 9 acetylation (H3K9ac), H3K14ac, and H3K27ac levels of Igf1 promoter region (n = 5); (B) The mRNA expression of albumin (Alb), and alpha-fetoprotein (Afp) (n = 5); (C) The mRNA expression (n = 5) and protein level (n = 3) of IGF1; (D) The mRNA expression of glucocorticoid receptor α (Gra), special protein 1 (Sp1), and P300 in the fetal liver (n = 8); (E) The mRNA expression of Grα, Sp1, and P300 in vitro (n = 5); (F) The protein level of GRα in vitro (n = 3); (G) The protein level of SP1 and P300 in vitro (n = 3); (H) The H3K27ac level of Igf1 promoter region (n = 3); (I) The Igf1 mRNA expression with GR, SP1, and P300 overexpression plasmid (n = 6). Mean ± S.E.M. ^* P < 0.05, ^** P < 0.01 vs. the control group. NS, no significance