Fig. 7

Expression of integrins at cell extremities and extracellular lipidosomes. A-F FEMX-I cells growing on fibronectin-coated supports were processed for CLSM. PFA-fixed and saponin-permeabilized cells were immunolabeled with various anti-integrin (ITG) antibodies as indicated and co-stained with fluorescent dyes LipidSpot™ 610 and fluorescence-conjugated WGA to highlight lipid droplets and glycoconjugates, respectively. Samples were counterstained with DAPI to highlight nuclei. Composite images of all x–y optical sections are shown and a given marker was pseudo-colored as indicated. Cells and extracellular lipidosomes are shown in the left and right panels, respectively, except for those in panel A’-F’. The yellow bracket indicates the very thin ITG β1⁺ process linking an extracellular lipidosome to the donor cell prior rupture, while arrowhead indicates specific ITG at cell extremities. Scale bars are indicated