Fig. 6

Cytoskeleton components associated with extracellular lipidosomes. A-C FEMX-I cells growing on fibronectin-coated supports were processed for CLSM. PFA-fixed and saponin-permeabilized cells were immunolabeled with anti-Vimentin, Nestin (A) or α-tubulin (C) antibodies or stained with fluorochrome-conjugated phalloidin (B). All samples were co-stained with the fluorescent dye BODIPY™ 493/503 and counterstained with DAPI to highlight lipid droplets and nuclei, respectively. Composite images of all x–y optical sections are shown. Cell extremities and extracellular lipidosomes are displayed in the left and right panels, respectively. The yellow bracket indicates the very thin Nestin⁺ process linking an extracellular lipidosome to the donor cell prior rupture (A). PC, phase contrast image. Scale bars are indicated