Fig. 4
Biogenesis of extracellular lipidosomes occurs during cell division or cell migration. A-D FEMX-I cells growing on fibronectin-coated supports were recorded in live by phase-contrast/fluorescence video microscopy after staining with LipidSpotâ„¢ 488/610. During cell division, cells can reabsorb material from their extremities (A, dashed line and white arrow) or lose it, resulting in the formation of extracellular lipidosomes (B, white arrowhead). During migration, cells can release extracellular lipidosomes (C, D, white arrowhead). Extracellular lipidosomes can be either large (B, C) or small (D). The very thin process linking an extracellular lipidosome to the donor cell can withstand traction before breaking (C, yellow bracket). Red arrow indicates the orientation of cell migration. Elapsed time in minutes is shown on the top-right corner. The images are excerpted from the Additional file 4: Video S3 (A, top), Additional file 6: Video S5 (B), Additional file 7: Video S6 (C) and Additional file 8: Video S7 (D). Scale bars are indicated