Fig. 5

Tumorous IRE1α-induced potentiated activation of CD8⁺T cells was mediated by NF-κB. A, E A2058 melanoma cells treated with HA15 (10 µM) for 24 h after pre-treated with or without STF-083010 (10 µM) or BAY 11-7085 (1 µM) for 24 h cocultured with or without activated T cell (1:3) for 24 h were subjected to crystal violet staining. Cytotoxicity was quantified by a spectrometer at OD (570 nm) and normalized ratio of cancer cell survival was shown for each well (n = 4). B-D, F-H Representative flow cytometry data and summary plots of the frequency of CD8⁺ T cells evaluated for expression of CD69, Granzyme B and IFN-γ in co-culture system with indicated treatment (n = 4). Data are representative of four independent experiments and shown as mean ± SD. Two-tailed Student’s t-test (*p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant)