Fig. 4

Tumorous IRE1α promotes the secretion of Th1-related chemokine and cytokines by activating NF-κB. A, D Immunoblotting analysis of IRE1α, p-IRE1α, p65, p-p65, XBP1s and GAPDH in A2058 cells treated with TG (0.5 µM) or HA15 (10 µM) for 24 h after pretreated with or without STF-083010 (10 µM), MKC8866 (0.5 µM) or IRE1α siRNA for 24 h. B-C, E-H Relative mRNA level (n = 6) and ELISA (n = 4) analysis of CXCL9, CXCL10, CXCL11, TNF-α and IL-6 in A2058 cells treated with TG (0.5 µM) or HA15 (10 µM) for 24 h after pretreated with or without STF-083010 (10 µM), MKC8866 (0.5 µM) or BAY 11-7085 (1 µM) for 24 h. I A2058 cells treated with TG (0.5 µM) or HA15 (10 µM) for 24 h after pretreated with or without MKC8866 (0.5 µM) for 24 h were subjected to ChIP with normal mouse IgG, NF-κB or Pol-II antibody as indicated (n = 3). J A2058 cells treated with TG (0.5 µM) or HA15 (10 µM) for 24 h were subjected to ChIP with normal mouse IgG, XBP1s or Pol-II antibody as indicated (n = 3). ChIP samples were analyzed by qPCR using primers indicated in Additional file 2: Table S3. Data are representative of at least three independent experiments and shown as mean ± SD. Two-tailed Student’s t-test or two-way ANOVA (*p < 0.05; **p < 0.01; ***p < 0.001)