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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Systematic assessment of chemokine ligand bias at the human chemokine receptor CXCR2 indicates G protein bias over β-arrestin recruitment and receptor internalization

Fig. 4

CXCR2 early endosomal trafficking. BRET signals were measured upon stimulation with increasing concentrations of CXCL1,-2,-3,-5,-6,-7 or CXCL8 of HEK293A cells transfected with the CXCR2.NLuc and FYVE.mNG. Results are expressed as % of endosomal detection whereby the maximal stimulation induced by CXCL8 was set as 100%. Data for the curves represents the Mean ± SEM, while the boxplot depicts the range from minimum to maximum, with a central line indicating the median across five independent experiments. Concentration-response curves were fitted to log (agonist) vs. response (three parameter) in GraphPad V9.3.1 (GraphPad Software, San Diego, CA, USA). Statistical analysis was done using one-way ANOVA followed by a Dunnet’s test. Significant differences were marked with *(P < 0.1), **(P < 0.01), ***(P < 0.001), or ****(P < 0.0001), non-significant differences were marked as ns. The dashed lines represent the mean value of CXCL8

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Cell Communication and Signaling

ISSN: 1478-811X