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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Systematic assessment of chemokine ligand bias at the human chemokine receptor CXCR2 indicates G protein bias over β-arrestin recruitment and receptor internalization

Fig. 2

CXCR2-mediated G protein activation profile. Chemokine (CXCL1,-2,-3,-5,-6,-7 and CXCL8) concentration-dependent BRET signals measured in HEK293A.CXCR2 cells transfected with the respective G protein biosensors. Results are expressed as % of G protein activation relative to the CXCL8 maximal response set at 100%. Gαi1, Gαi2, Gαi3, GαoA, GαoB, and Gα15 activation data is shown in panels A to F, respectively. Data of the curves represents Mean ± SEM, while the boxplot depicts the range from minimum to maximum, with a central line indicating the median across five to six independent experiments. Concentration-response curves were fitted to log (agonist) vs. response (three parameter) model in GraphPad V9.3.1 (GraphPad Software, San Diego, CA, USA). Statistical analysis used one-way ANOVA with Dunnet's test (*P < 0.05, ns for non-significant differences), and dashed lines indicate the mean value of CXCL8 for reference

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Cell Communication and Signaling

ISSN: 1478-811X