Fig. 1

Competition binding assay shows that all ELR + chemokines are high-affinity ligands for CXCR2. A competition binding assay was performed on HEK293A.CXCR2 cells incubated with CXCL8^AF647 (25 ng/mL) and increasing concentrations of unlabeled CXCL1-3 and CXCL5-8 as competitors. A Concentration-response curves showing CXCL8^AF647 binding inhibition by unlabeled chemokines. Data are normalized using a vehicle control and show the Mean ± SEM (n = 5). B To determine the pKi values, concentration-response curves were fitted to one site – Fit Ki in GraphPad V9.3.1 (GraphPad Software, San Diego, CA, USA). Statistical analysis of the pKi values was performed using one-way ANOVA followed by a Dunnet’s test with CXCL8 as the reference ligand (pKi of CXCL8 is represented by the dashed line). Statistical significance is marked as **** (P < 0.0001) and *(P < 0.1), while non-significant differences are marked as ‘ns’