Fig. 1

Experimental protocol and effect of H₂S on spinal cord structure and lower limb motor function in SCIRI rats. a Schematic of the timeline of SCIRI induction, GYY4137 administration, and subsequent analysis. b Representative spinal cord cross-section Nissl staining showing the ventral horn regions of the spinal cord (100x, scale bar = 200 μm). c Quantitative analyses of Nissl body in the ventral horn regions of the spinal cord (n = 3 rats per group). d The BBB scores of different groups at each time point (n = 14 rats per group). Data are presented as mean ± SD. *p < 0.05; ***p < 0.001. BBB, Basso, Beattie & Bresnahan locomotor rating scale; PI, propidium iodide; SCIRI, spinal cord ischemia‒reperfusion injury; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling