Fig. 8

Permeability and lymphocyte adhesion molecule expression of HMVEC is not increased by C-terminally truncated CXCL10_(1–73) or intact CXCL10_(1–77). A Endothelial monolayer permeability was assessed after stimulation with control medium (CO), or stimulated with VEGF (100 ng/ml) alone or VEGF (100 ng/ml) combined with 360 nM CXCL10_(1–77) or CXCL10_(1–73). Data are displayed as median (± IQR) of 4 to 6 independent experiments. Mann–Whitney U test was performed (* p ≤ 0.05, ** p ≤ 0.01 for comparison to control). Expression and/or MFI of B PECAM-1/CD31, C, D ICAM-1/CD54, and E, F VCAM-1/CD106 on HMVEC (gated as CD31⁺ cells) was evaluated through flow cytometry upon stimulation for 48 h at 37 °C and 5% CO₂ with control medium (CO), 100 ng/ml TNF-α and 100 ng/ml IFN-γ, or CXCL10_(1–77) or CXCL10_(1–73) at the indicated doses. The data are displayed as median (± IQR) of 4 to 6 independent experiments. Mann–Whitney U test was performed (* p ≤ 0.05, ** p ≤ 0.01 for comparison to control)