Fig. 7

NET elimination alleviates EAE severity through reduced recruitment of Th1 and Th17 cells. A–E EAE severity and T cell recruitments were evaluated in control and DNase-1 groups. The DNase-1 group received DNase-1 from post- 7 days induction to peak of the disease, whereas the control was inoculated with the vehicle. A Clinical scores comparison between the control and DNase-1 groups. B Tracking of changes in body weight, following the administration in both groups. Both groups (A and B) were comprised of 10 mice each. C And D A comparison of demyelination degree in both groups, stained with luxol fast blue. C Upper, representative images of brain sections. Lower, the demyelinated area in the corpus callosum of the brain was presented. Scale bar = 500 μm. D Left, representative images of spinal cord sections. Scale bar = 500 μm. Right, the analysis of demyelination degree in both groups through thoracic to lumbar part of spinal cord. E and F Analysis of T cell populations, particularly CD4⁺IFNγ⁺, CD4⁺IL-17A⁺, and CD4⁺IFNγ⁺IL-17A.⁺ cell population, through flow cytometry in the (E) brain and (F) spinal cord collected from both groups. Data are obtained from at least three independent experiments and presented as mean ± SEM. *P < 0.05, **P < 0.01, ****P < 0.0001