Fig. 5

NLRP3 enhances CXCR2 and CXCR4 expression and degranulation in neutrophils, supporting NET formation during EAE pathogenesis. A–D NET-associated neutrophil phenotypical characteristics were compared between WT and NLRP3 KO mice. Analysis of the relative MFI of extracellular CD11b, CXCR2, and CXCR4 on neutrophils (gated on CD11b⁺ and Ly6G⁺ cells) from the (A) brain and (B) circulating neutrophils in both groups, as shown by representative histogram. Representative histogram and relative MFI of extracellular CD63 on neutrophils (gated on CD11b⁺ and Ly6G.⁺ cells) from both groups at the disease peak in the (C) brain and (D) circulating neutrophils. ns, non-significant. Data are obtained from at least three independent experiments and presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001