Fig. 4
From: NLRP3 exacerbates EAE severity through ROS-dependent NET formation in the mouse brain
NLRP3 facilitates NET accumulation in the brain during EAE progression. A Immunoblotting and densitometric analysis for MPO, CitH3, and β-actin in the brain from WT and NLRP3 KO mice at peak disease. For quantification, the target expressions were normalized using β-actin. B Representative fluorescent images of NET formation in the brain from both groups, harvested at peak disease. Fluorescence images were acquired by staining for MPO (green), CitH3 (red), and nuclei (blue). Scale bar = 50 μm. Representative data from at least three independent experiments are shown. Data are driven from triple repeats and displayed as mean ± SEM. *P < 0.05, ***P < 0.001