Fig. 3

NLRP3 enhances NET formation predominantly in the brain of EAE pathogenesis. A–D Comparison of relative expression of NET components and percentage of NET formation in the brain and spinal cord of WT and NLRP3 KO mice at the disease peak. Representative histogram and relative MFI of extracellular MPO or CitH3, gated on CD11b⁺ and Ly6G⁺ cells, in both groups at peak disease on the (A) brain-infiltrated and (C) spinal cord-infiltrated neutrophils. Comparison of NET formation (Ly6G⁺CitH3⁺MPO.⁺) percentage in the (B) brain and (D) spinal cord from both groups. ns, non-significant. Data are acquired more than triplicates reiteration and presented as mean ± SEM. *P < 0.05, **P < 0.01