Fig. 2

NLRP3 exacerbates EAE severity, but is irrelevant to neutrophil infiltration into the CNS during EAE peak. A–F Comparison of EAE severity and CNS-infiltrated neutrophil number between WT and NLRP3 KO mice. A Clinical scores of EAE from both groups. The combined results of three independent experiments are shown. B Relative body weight in both groups following EAE induction. Both groups (A and B) were conducted with 15 mice each. (C and D) A comparison of brain demyelination between both groups at the peak of the disease, stained with luxol fast blue staining. C Upper, representative images of brain sections. Lower, the demyelinated area in the corpus callosum of WT and NLRP3 KO brains. D Left, representative images of, spinal cord demyelination in both groups at the disease peak. Scale bar = 500 μm. Right, the analysis of demyelination degree in both groups through thoracic to lumbar part of spinal cord. Representative scatter plots and the absolute number of infiltrated neutrophils in the E brain and F spinal cord of both groups at peak disease. ns, non-significant. Data are driven from at least triple replicates and presented as mean ± SEM. *P < 0.05, ***P < 0.001, ****P < 0.0001