Fig. 1
From: NLRP3 exacerbates EAE severity through ROS-dependent NET formation in the mouse brain
Neutrophils and their extracellular traps were substantially observed in the CNS during EAE pathogenesis. A–F Identification of neutrophil infiltration and NET formation in the EAE-induced CNS, as compared to control. Representative scatter plots and the absolute number of infiltrated neutrophils in the (A) brain and (B) spinal cord. Immunoblotting and densitometric analysis for MPO, CitH3, and β-actin in the (C) brain and (D) spinal cord. For quantification, the target expressions were normalized using β-actin. Representative histogram of the percentage of NET formation (Ly6G+CitH3+MPO.+) and graph in the (E) brain and (F) spinal cord. Cont, control. Data are obtained from at least triplicated repeat and presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001