Fig. 3
From: NPFs-mediated actin cytoskeleton: a new viewpoint on autophagy regulation
The model for the activation of the Arp2/3 complex mediated by NPFs and the formation of the branched actin network mediated by NPFs. NPFs lead to conformational change and activation of the Arp2/3 complex. Class I NPFs bring the activated Arp2/3 complex together with the actin mother filaments via WCA, mediating the nucleation of daughter filaments. Class II NPFs stabilize Arp2/3 branchpoints and alter the conformation of F-actin to increase the affinity of the Arp2/3 complex for actin mother filaments