Fig. 2
From: NPFs-mediated actin cytoskeleton: a new viewpoint on autophagy regulation
Structural organization of NPFs. Class I NPFs contain WASP, WASH, WAVE, WHAMM, and JMY, which have characteristic WCA domains at the C-terminal. WCA is the smallest unit of the efficient activation of the Arp2/3 complex, with the WH2 domain capable of binding to G-actin and the CA domain responsible for binding to Arp2/3. The main member of class II NPFs is cortactin, without WCA domains. NTA domain binds to the Arp2/3 complex, and the tandem repeat domain binds to F-actin. The N-terminal domains of NPFs are different and can provide binding sites for a wide range of regulatory factors in cells. WH1, WASP homology1; WAHD, WASH homology domain; WHD, WAVE homology domain; WMD, WHAMM membrane interaction domain; LIR, LC3-interacting region; GBD, GTPase binding domain; PRD, proline-rich-domain; CC, coiled-coil; W, WASP homology2 (WH2) domain; C, central domain; A, acidic domain; NTA, N-terminal acidic domain; SH3, Src homology 3 domain