Fig. 6
miR155-3p promoted NLRP3-mediated pyroptosis in H/R injury. HK-2 cells were seeded at high density (125,000 cells/cm2, GJ formed) and subjected to H/R. A The survival rate of HK-2 cells in different groups. Con: control group; Mic: mimic of miR155-3p (50 nM) transfected into cells for 48 h before the next step; IN: inhibitor of miR155-3p (100 nM) transfected into cells for 48 h before the next step. * p < 0.05 vs. control; # p < 0.05 vs. H/R. Data are means ± SEM (n = 5). B Concentration of IL-18 and IL-1β in culture supernatant in HK-2 cells of different groups. * p < 0.05 vs. control; # p < 0.05 vs. H/R. Data are means ± SEM (n = 5). C The expression of NLRP3, GSDMD, GSDMD-N, IL-18, and mature IL-18 in HK-2 cells were analyzed by western blots. * p < 0.05 vs. control; # p < 0.05 vs. H/R. Data are means ± SEM (n = 3)