Fig. 5

USP22 sustains the OXPHOS-potential of HER2⁺-BC and TNBC cells: A Schematic representation of the TNBC and HER2⁺-BC models utilized to study the dependency of OXPHOS on USP22. B-E Real-time quantitative PCR (RT-qPCR) (B-C), measurement of the oxygen consumption rate (OCR; D) and mitochondria staining using mitotracker (E) performed on TNBC and HER2⁺-BC models strongly present a downregulation of several OXPHOS-related genes, impaired OCR and altered mitochondria morphology upon USP22 depletion. E–F: White scale bar: 20 µm. F-G Mitotracker staining (F) and OCR measurement (G) in veh- and USP22i-S02-treated HCC1806 cells. Statistics: B, C: Student t-test; D, G: Student t-test (based on the area under the curve = AUC); E–F (right panel): non-parametric Mann–Whitney test. *p<0.05, **p<0.01, ***p<0.005. All experiments were performed in at least three biological replicates