Fig. 3

USP22 supports the tumorigenic properties of TNBC cells. A-D Growth kinetics (A), colony formation assay (B), tumorsphere formation assay (C) and gap closure assay (D) strongly point to a loss of the tumorigenic properties upon USP22 silencing in TNBC cells. E–H Growth kinetics (E), colony formation assay (F, 25 µM), tumorsphere formation (G) and gap closure assay (H, 50 µM) of DMSO (veh)- and USP22i-S02-treated TNBC cells. Statistics: A, D, E and H (based on the area under the curve = AUC), B-C, G right panels: Student t-test; B-C, F-G left panels: non parametric Mann Whitney test; *p<0.05, **p<0.01, ***p<0.005. All experiments were performed in at least three biological replicates