Fig. 6

Ferritin promotes the production of NETs by activating Msr. A Heatmap of differentially expressed mRNA from BMDNs extracted for RNA-seq after ferritin stimulation applied to mice. B and C WB and qPCR results of Msr protein and mRNA levels in BMDNs. D Msr protein expression levels in lung tissues. E and F Proportion of neutrophils in peripheral blood and lungs of mice after ferritin stimulation. G and H Mean fluorescence intensity of MSR and percentage of Msr-positive cells in ferritin-treated human neutrophils. I-J DNA concentration and MPO-DNA concentration after ferritin injection using the Msr inhibitor Fucodin combined with ferritin. K IF images of BMDNs in mice after ferritin injection using the Msr inhibitor Fucodin combined with ferritin (MPO, red; CitH3, green; DAPI, blue. Scale bar = 20 μm). L and M Morphological and HE-stained images of lungs in mice. N IF images of CitH3 in lung tissue of WT and Msr ablated mice treated with ferritin (CitH3, red; DAPI, blue. Scale bar = 25 μm). O IF staining and (P) MPO-DNA complex concentration of BMDNs from ferritin-treated WT and Msr−/− mice with PMA stimulation in vitro