Fig. 5

Bone marrow of donors with PAD4, NE, ROS gene defects inhibited ferritin-induced NET production in WT recipient. A Overview of the experiment: WT, Padi4−/−, Elane−/−, and Cybb−/− mice had their bone marrow transplanted into WT recipients. The recipients were treated with ferritin after 4 weeks. B IF staining of BMDNs after ferritin injection in bone marrow transplanted mice (MPO, green; CitH3, red; DAPI, blue. Scale bar = 40 μm). C Scanning electron microscope images of BMDNs (Scale bar = 10 μm). D Peripheral blood DNA concentration. E and F Peripheral blood and lung neutrophil frequency. G-I Serum inflammatory factors (IL-6, MCP-1 and TNF-α) levels. J and K Lung wet-to-dry ratios and lung injury scores. L and M HE staining and Masson staining images of lung tissue. N and O Inflammatory factor-associated mRNA levels in lung tissue