Fig. 3

Ferritin-induced systemic inflammatory response and lung injury were dependent on neutrophils. Co-administration of anti-Ly6G antibody to ferritin-treated mice for 12 h. A, B, C and D Neutrophil number, frequency, monocyte frequency and Ly6C+ neutrophil frequency in mouse lungs. E Lung injury score. F HE staining of 12 h mouse lung tissue (Scale bar = 100 μm), SEM images of BMDN neutrophil NETs (Scale bar = 10 μm), and IF images of lung tissue (Ly6G, green; CitH3, red; DAPI, blue. Scale bar = 30 μm). G lung wet to dry rate. H The proportion of cells releasing NETs in peripheral blood. I The proportion of NETs-positive cells in lung tissue. J, K, L, M and N Mouse plasma levels of inflammatory factors: IL-6, TNF-α, MCP-1, IL-10 and IFN-γ. O Immunoblot of mouse lung tissue for CitH3 protein expression. P, Q and R Il1b, Il6, Tnf mRNA levels in lung tissue. *p < 0.05