Fig. 2

Ferritin promotes the formation of NETs. Neutrophils isolated from ferritin-treated and control mice were treated with PMA. A Representative immunofluorescence staining of spontaneous, PMA-induced NET formation in ferritin-treated mice at 12 h BMDNs. Neutrophils were stained with MPO (green), CitH3 (red) antibodies and DAPI (blue). Scale bar, 30 μm. B Serum MPO-DNA complexes and (C) dsDNA concentration in cell culture supernatant. D Heatmap of RNA-seq differential analysis of BMDNs in PBS and ferritin-injected mice. E Immunofluorescence staining images of mice lung tissue after PBS or ferritin treatment. (MPO, green; CitH3, red; DAPI, blue. Bar = 30 μm). F Image of SEM (scanning electron microscopy) showed the morphology of extracellular meshes NETs formation after ferritin injection. (bar = 10 μm) *p < 0.05