Fig. 1

Ferritin induces systemic inflammation and lung injury in vivo, resulting in lung neutrophil accumulation. Mice were treated with ferritin injections for the corresponding times (3 h, 6 h, 12 h, 24 h, 48 h). A Lung tissues’ HE staining of ferritin-injected mice. B Lung injury scores of ferritin-treated mice (n = 6). C and D Neutrophils frequency and cell number in peripheral blood of mice (n = 6). E Lung wet to dry rate (n = 6). F and G Frequency and number of peripheral blood monocytes. H and I Frequency and number of Ly6C+ monocytes in peripheral blood (n = 6). J Splenic appearance of mice 12 h after ferritin treatment. K, L, P, and Q Plasma inflammatory factor levels in mice (IL-6, TNF-α, IL-1β, and MCP-1, n = 4). M, N, O, R, S, and T Lung tissue neutrophil, monocyte, Ly6C+ monocyte, macrophage, T-cell, and B-cell frequencies (n = 6) *p < 0.05