Fig. 7
From: Regulation of β-cell death by ADP-ribosylhydrolase ARH3 via lipid signaling in insulitis
Protective effect of ω-3 fatty acids against cytokine-induced apoptosis. Apoptosis was measured by caspase3/7 activity in MIN6 cells treated with ω-3 fatty acids or ethanol (Eth) and PLA2G6 siRNA (siPLA2G6) for 48 h followed by 24 h of cytokine cocktail CT2 (IL-1β + IFN-γ + TNFα) treatment (n = 4, +SD). *p ≤ 0.05, **p ≤ 0.01, *** p ≤ 0.001 and ****p ≤ 0.0001 by 2wayANOVA and “Uncorrected Fisher’s LSD” test. Specifically, the normality and outlier test for the molecular experiment were tested using “The shapiro-Wilk test” and Dixon’s test, with a threshold of p < 0.2, respectively