Fig. 3
From: Regulation of β-cell death by ADP-ribosylhydrolase ARH3 via lipid signaling in insulitis
Pro-inflammatory cytokines and Pla2g6-dependent proteome remodeling in MIN6 cells. a Cytokine cocktail CT2 (IL-1β + IFN-γ + TNFα)-dependent protein expression in Pla2g6 siRNA (siPla2g6) MIN6 cells (n = 5, +SD). Nontarget siRNA was used as a transfection control. b KEGG pathways enriched with proteins differentially abundant in IL-1β + IFN-γ-treated MIN6 cells. Pathways were grouped based on shared proteins using Enrichment Map tool in Cytoscape [30]. Each pathway is represented by a node, and their degree of connectivity (thickness of the edges) is proportional to the number of shared proteins between the pathways. c Pla2g6-dependent protein abundance changes of CT2-treated MIN6 cells. Abundance profiles of cathepsin Z (d), cathepsin B (e). Statistical test: ** p ≤ 0.01 and *** p ≤ 0.001 by 2way-ANOVA and “Šídák’s multiple comparisons test