Fig. 2
From: Regulation of β-cell death by ADP-ribosylhydrolase ARH3 via lipid signaling in insulitis
Spatial localization of lysophosphatidylcholines, phosphatidylcholines and phospholipase PLA2G6 in pancreata. a Chemical image of mouse and human pancreata by mass spectrometry. Each image shows either the optical image or color-coded distribution of different lipids. Lipid species were identified by matching against the lipids characterized and quantified on the lipidomics analysis based on their accurate masses. b PLA2G6 fluorescence in situ hybridization (FISH) of islets from non-obese diabetes resistant (NOR) mice (6 weeks of age) and MIN6 cell line. Cells and tissues were stained with anti-insulin antibody (green), DNA stain 4′,6-diamidino-2-phenylindole (DAPI – blue) and fluorescent-labeled antisense Pla2g6 oligonucleotide (red). c Immunohistochemistry (IHC) analysis of PLA2G6. Tissue was stained with biotin-conjugated anti-Pla2g6 antibodies followed by avidin-conjugated horseradish peroxidase. Localization was visualized by horseradish peroxidase-mediated oxidation and precipitation of 3,3′-diaminobenzidine (brown). The images are representative of two independent experiments