Fig. 1
From: Regulation of β-cell death by ADP-ribosylhydrolase ARH3 via lipid signaling in insulitis
Global lipidomic analysis of 3 common insulitis models, i.e., EndoC-βH1 (n = 3) cells and human islets (n = 10) exposed to CT1 (IL-1β and INF-γ) for 48 h and islets from non-obese diabetic (NOD) mice in pre-diabetic stage (6 weeks of age) vs. age-matched NOR mice (n = 3). Lipids were extracted and analyzed by liquid chromatography-tandem mass spectrometry. a Volcano plots of the lipid species relative abundances. b Number of lipid species significantly (Student’s t-test p ≤ 0.05) regulated in each class. “α” represents common lipid species upregulated or downregulated in all three insulitis models. c Lipid species that are consistently regulated in the 3 insulitis models. Each lipid species is named with the abbreviation of its class (e.g., LPC and PC) followed by the length of the fatty acid and number of double bonds (separated by a colon) in parenthesis. The letters after the lipid names represent different isomers that are separated in the chromatography in alphabetical order. The relative abundance in T1D model vs. control is color-coded. Isobaric coeluting species (separated by semicolons) were co-quantified