Fig. 6

KinasePredictor validation using well-established phosphorylation sites. Three sequences with experimentally verified phosphorylation sites from PhosphoSitePlus were input into KinasePredictor, ELK1 at S384, a known target of Erk1/2 (A, left), IκB1α at S293, a known target of CK2α1 (B, mid), and TSC2 at S1388, a known target of AMPKα1/2 (C, right). For each sequence, a dot product was calculated for each kinase in the dataset, ranked, and then graphed with their dot product value. The lowest rank and highest dot product kinases represent those predicted most likely to phosphorylate the target site given the input sequence. The kinases known to target the site are indicated on each graph and their rank shown in parentheses. Kinases are color-coded by their family according to the legend on the right. Kinase groups are AGC, PKA/PKG/PKC family; CAMK, calmodulin-kinase family; CK1, casein kinase family; CMGC, cyclin-dependent kinase/mitogen-activated kinase/glycogen-synthase kinase/CDK-like kinase family; STE, sterile family kinases; TK, tyrosine kinases; TKL, tyrosine-like kinases