Fig. 3

Comparison of substrate logos of representative kinases from two studies using different profiling methods: in vitro phosphorylation/phosphoproteomics technique (“Mass Spectrometry”) (left) and combinatorial peptide library screening method (“Peptide Array”) in which a library of target peptides is phosphorylated in vitro (right) [16]. Representative targets were chosen from different kinase families. In the logo images in the “Mass Spectrometry” column, the colors of individual amino acids indicate different side chain properties: blue, basic; red, acidic; green, hydrophobic; black, aromatic; magenta, polar/uncharged; orange, no side chain. The logo images in the “Peptide Array” column are from supplementary files from Johnson et al. [16] and use a similar format to what was used in the “Mass Spectrometry” column except that the anti-logos are given as downwardly directed stacks. These logos were copied directly from reference 14. (Reference 14 was licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as if appropriate credit is given to the original author(s) and the source. Creative Commons License: https://creativecommons.org/licenses/by/4.0/)