Fig. 2

Comparison of substrate logos of representative kinases from two in vitro kinase profiling studies using MS-based methods, Sugiyama et al. 2019 [13] (left), and Douglass et al. 2012 [12] (right). Kinases are representatives from the indicated protein kinase families. Logos were generated in PTM-Logo (Chi squared filtering α = 0.0001) from “n” number of input peptide sequences for each kinase. Dendrograms were made using Interactive Tree of Life (iToL). The colors of individual amino acids indicate different side chain properties: blue, basic; red, acidic; green, hydrophobic; black, aromatic; magenta, polar/uncharged; orange, no side chain