Table 1 List of antibodies used in this study

anti-Nestin

Wako

012–26843

1:200 dilution (IF)

anti-NeuN

Abcam,

ab279296

1:400 dilution (IF)

anti-NeuN

Sigma

ABN78A4

1:100 dilution (IF)

anti-GFAP

Abcam

ab53554

1:800 dilution (IF)

1:1000 dilution (WB)

anti-Tuj-1

Biolegend

801202

1:200 dilution (IF)

1:1000 dilution (WB)

anti-L1CAM

Proteintech

67115-1-Ig

1:200 dilution (IF)

1:2000 dilution (WB)

anti-CD31

R&D

FAB3628G

1:200 dilution (IF)

anti-CD31

Proteintech

65058-1-Ig

1:100 dilution (IF)

anti-TSG101

Proteintech

28283-1-AP

1:200 dilution (IF)

anti-SOX2

Abcam

ab171380

1:200 dilution (IF)

anti-O4

Sigma

MAB345M

10 μg/mL (IF)

anti-Akt

Wanlei

WL0003b

1:500 dilution (WB)

anti-p-Akt

Proteintech

66444-1-Ig

1:5000 dilution (WB)

anti-mTOR

Wanlei

WL02477

1:500 dilution (WB)

anti-p-mTOR

Proteintech

67778-1-Ig

1:2000 dilution (WB)

Alexa Fluor® 488 Donkey Anti-Mouse IgG

Abcam

ab150105

1:800 dilution (IF)

Alexa Fluor® 647 Donkey Anti-Mouse IgG

Abcam

ab150107

1:800 dilution (IF)

Alexa Fluor® 488 Donkey Anti-Rat IgG

Abcam

ab150153

1:800 dilution (IF)

Alexa Fluor® 594 Donkey Anti-Rat IgG

Abcam

ab150156

1:800 dilution (IF)

Alexa Fluor® 594 Donkey Anti-Rabbit IgG

Abcam

ab150076

1:800 dilution (IF)

Alexa Fluor® 594 Donkey Anti-Goat IgG

Abcam

ab150136

1:800 dilution (IF)

Alexa Fluor® 488 Donkey Anti-Rabbit IgG

Abcam

ab150073

1:800 dilution (IF)

Alexa Fluor® 647 Donkey Anti-Goat IgG,

Abcam

ab150135

1:800 dilution (IF)

Alexa Fluor® 488 Donkey Anti-Goat IgG

Abcam

ab150129

1:800 dilution (IF)

Goat anti-rabbit IgG

Proteintech

SA00001-2

1:5000 dilution (WB)

Goat anti-mouse IgG

Proteintech

SA00001-1

1:5000 dilution (WB)

Rabbit anti-goat IgG

Proteintech

SA00001-4

1:5000 dilution (WB)