Fig. 7

UTX KO SCMECs-derived EVs promotes neural differentiation through L1CAM-Akt/mTOR pathway. A Western blotting analysis of the expression levels of the L1CAM proteins, Akt/mTOR signaling pathway-related proteins, neuron marker protein Tuj-1 and astrocyte marker protein GFAP in KO EVs group, NC EVs group and PBS group. B, C Statistical analysis of Tuj-1 and GFAP expression in each group in figure A, n = 3 per group. D Western blotting analysis of the expression levels of the L1CAM proteins, Akt/mTOR signaling pathway-related proteins, Tuj-1 proteins and GFAP proteins in KO EVs plus Akt inhibitor intervention group NSCs, KO EVs plus L1CAM shRNA intervention group NSCs and KO EVs without other intervention group NSCs. E, F Statistical analysis of Tuj-1 and GFAP expression in each group in figure D, n = 3 per group. G Immunofluorescent analysis of the neural differentiation and astrocytic differentiation of NSCs in vitro in KO EVs plus Akt inhibitor intervention group NSCs, KO EVs plus L1CAM shRNA intervention group NSCs and KO EVs without other intervention group NSCs. Scale bar, 40 μm. H, I Statistical analysis of Tuj-1⁺ cells and GFAP⁺ cells to all cells in figure G, n = 10 per group. ^nsP > 0.05, *P < 0.05, **P < 0.01, compared with corresponding control group