Fig. 6

UTX KO SCMECs EVs promote neural differentiation. A Immunofluorescent identification of tdToamto⁺ NSCs (red) phagocytize KO EVs (green) labeld by PKH67. Scale bar, 40 μm. B Immunofluorescent analysis of the neural differentiation of tdToamto⁺ NSCs and expression of L1CAM in KO EVs group, NC EVs group and PBS group. Scale bar, 40 μm. C Statistical analysis of the ratio of NeuN⁺tdtomato⁺ cells to tdTomato⁺ cells in figure B, n = 6 per group. D Immunofluorescent analysis of the astrocytic differentiation of tdToamto⁺ NSCs and expression of L1CAM in PBS group. Scale bar, 40 μm. E, F colocalization analysis of GFAP channel and L1CAM channel in figure D. Scale bar, 40 μm. G Immunofluorescent analysis of the neural differentiation and astrocytic differentiation of NSCs in KO EVs group, NC EVs group and PBS group. Scale bar, 40 μm. H, I Statistical analysis of Tuj-1⁺ cells and GFAP⁺ cells to all cells in each group in figure G, n = 10 per group. ^nsP > 0.05, *P < 0.05, **P < 0.01, compared with corresponding control group