Fig. 4

SCMECs were in the high EVs secretion state and Inhibition of EVs secretion hindered neural differentiation. A EB permeation assays of UTX KO mice and NC mice in the sham group and 3 days after SCI. Scale bar, 100 μm. B Immunofluorescent analysis of the expression of TSG101 (red) in UTX KO SCMECs (green) and NC SCMECs (green) in sham group and 3 days after SCI. Scale bar, 20 μm. C Statistical analysis of the ratio of TSG101⁺CD31⁺ cells to CD31⁺ cells in figure B, n = 6 per group. D The immunofluorescence analysis of the neural differentiation in NSCs lineage-traced mice 14 days post-SCI after GW4869 intervention. Scale bar, 20 μm. E Statistical analysis of the ratio of NeuN⁺tdtomato⁺ cells to tdTomato⁺ cells in figure D, n = 6 per group. F Distribution of the BMS scores over time post-SCI in GW4869 and Control groups. G Immunofluorescent analysis of the neural differentiation of NSCs co-cultured with UTX KO SCMECs intervened by GW4869 (GW4869 group) or PBS (Control group). Scale bar, 40 μm. H Statistical analysis of Tuj-1⁺ cells to all cells in each group in figure G, n = 10 per group. I Immunofluorescent analysis of the neural differentiation of NSCs co-cultured with NC SCMECs intervened by GW4869 or PBS (Control group). Scale bar, 40 μm. J Statistical analysis of Tuj-1⁺ cells to all cells in each group in figure I, n = 10 per group. ^nsP > 0.05, *P < 0.05, **P < 0.01, compared with corresponding control group