Fig. 3

UTX KO SCMECs highly expressed L1CAM. A Volcano plot of differentially expressed mRNA in UTX KO SCMECs compared to NC SCMECs. B Hierarchical cluster heatmap of the differential mRNAs in UTX KO SCMECs compared to NC SCMECs. C Dot plot of GO enrichment analysis. D Fold change of neurogenesis related genes in SCMECs after UTX KO. E Immunofluorescent analysis of the expression of L1CAM (red) in UTX KO SCMECs (green) and NC SCMECs (green) on the 3rd day of SCI. Scale bar, 40 μm. F Statistical analysis of the ratio of L1CAM⁺CD31⁺ cells to CD31⁺ cells in figure E, n = 6 per group. G Fluorescence in situ hybridization analysis of the expression of L1CAM mRNA (green) in UTX KO SCMECs (red) on the 3rd day of SCI. Scale bar, 20 μm. H Western blotting analysis of the expression levels of the L1CAM proteins in UTX KO SCMECs and NC SCMECs. I Statistical analysis of L1CAM expression in each group in figure H, n = 3 per group. ^nsP > 0.05, *P < 0.05, **P < 0.01, compared with corresponding control group